喉癌间质肥大细胞的形态学特点及分布意义的探讨

THE DISTRIBUTION SIGNIFICANCE AND MORPHOLOGICAL STUDY OF MAST CELLSIN THE STROMA OF LARYNGEAL CARCINOMA

应用免疫组织化学和透射电镜技术对 45例喉鳞癌组织 (其中 35例取癌中心为癌组 ,另 10例取癌旁组织为癌旁组 )中的肥大细胞 (MC)进行研究。结果 :1.癌旁组织中有丰富的 MC浸润而癌间质中 MC脱颗粒明显 ;2 .阿尔辛蓝·藏红 (AB· S)染色 ,二组 MC的组化性质显示出了差异 ;3.喉癌细胞增殖细胞核抗原 (PCNA)的阳性表达与 MC分布之间存在一定的相关性 ;4.癌间质 MC属类胰蛋白酶及糜蛋白酶 (TC)型 ,常与癌细胞有紧密接触 ,并可见 MC颗粒脱向癌细胞。结果提示 :MC与癌细胞关系密切 ,可能有抑制癌细胞增殖的倾向。

The mast cells in forty- five cases of laryngeal squamous carcinoma were investigated immunohistochemically and electro- microscopically.They were divided into two groups:the mast cells in the stroma of the cancer ( 35cases) and those distributed in the periphery tissue around the cancer ( 1 0 cases) .The results were as follows 1 .There were many mast cells infiltrated in the peritumoral stroma and in the tumor stroma degranulation was significant.2 .Mastcells in the tumor stroma and peritumoral stroma showed different chemical feature when stained with Alcian blue- safranin.3.The expression of PCNA- positive cells exhibited a certain correlation with the distribution of infiltration mast cells in the stroma of laryngeal carcinoma.4.Mast cells in the tumor stroma belonged to tryptase and chymase mast cell.It was observed that mast cells often contacted with the cancer cells and with its granules'content releasing to the cancer cells.Our results suggest that there is a close relationship between mast cells and cancer cells,Mastcells may play roles in inhibiting cancer cells’ proliferation.