间隙连接蛋白基因connexin43、ER、PR的表达在子宫平滑肌瘤中相关性研究

THE RELATIONSHIP OF EXPRESSION OF CONNEXIN 43 mRNA,Cx43,ESTROGEN RECEPTOR AND PROGESTERONE RECEPTOR IN HUMAN LEIOMYOMA AND MYOMETRIUM TISSUES

研究细胞间隙连接蛋白基因 43(connexin43,CX43)及其蛋白、雌激素受体 (estrogen receptor,ER)、孕激素受体 (progesterone receptor,PR)在子宫平滑肌瘤中的表达 ,从核酸及蛋白水平探讨在子宫平滑肌瘤发生中的相关关系。应用核酸原位杂交技术和 SP免疫组织化学法 ,研究 37例子宫平滑肌瘤、 2 0例正常子宫平滑肌组织中 cx43m RNA及其蛋白、ER、 PR的表达规律。结果显示 ,cx43m RNA及其蛋白、 ER、 PR在子宫平滑肌瘤中的表达明显高于在子宫平滑肌组织中的水平 ,差异有显著性 (P<0 .0 5 )。 cx43m RNA及其蛋白在子宫平滑肌瘤中的过度表达 ,是子宫平滑肌瘤发生过程的重要事件 ,与 ER、 PR水平升高呈现一致性 ,对进一步揭示子宫平滑肌瘤的复杂分子机制、寻求可靠的早期标志有重要意义。

To investigate the relationships of the expressions and the roles of connexin 43 mRNA and its protein Cx43, estrogen receptor(ER) and progesterone receptor (PR) in human leiomyoma and myometrial tissues from the level of nuclear acid and protein, in situ hybridization and immunohistochemical techniques were employed to detect the levels of cx43 mRNA, Cx43, ER and PR in 37 cases of leiomyoma tissues and 20 cases of myometrium tissues. The results showed that steady state and higher levels of cx43 mRNA, Cx 43 ER and PR proteins were detected in leiomyoma tissues from nonpregnant premenopausal women, which had a significant higher positive rates compared with those in myometrium tissues( P <0 05). The conclusion might be that the increase of cx43 mRNA and Cx43 protein might play an important role in leiomyma. Differences in the levels of cx43 expression in leiomyma and myometrium might reflect defferences in responses to steroid hormones. \;