端粒酶RNA反义基因对肝癌细胞的影响

THE EFFECT OF HUMAN TELOMERASE ANTISENSE RNA ON LIVER CANCER CELLS

用RT-PCR的方法钓取端粒酶RNA基因的cDNA,并将其反向插入到逆转录病毒载体pLNCX上,构建hTR基因的反义表达质粒。将质粒经脂质体介导转染人肝癌SMMG-7721细胞中表达。结果表明hTR反义基因的表达有效地封闭或抑制肝癌细胞的端粒酶活性,抑制细胞的生长和增殖,延长细胞的倍增时间并促进细胞凋亡。

The cDNA of hTR( human telomerase RNA)gene was cloned by the means of RT-PCR and inserted into the reverse transcription virus vector(PLNCX)to construct the antisense expression plasmid of hTR gene. By using lipofectin-mediated DNA transfection, the ob- rained plasmid WPS successfully expressed in human liver cancer cells (SMMC-7721). All data suggest that telomerase antisense RNA could significantly inhibit the telomerase activity and proliferation of cells,prolong cell's doubling time and induce the apotosis of cells.