昆明地区菊花病毒病的调查与鉴定

Survey and Identification of Chrysanthemum Virus Diseases in Kunming

从昆明地区菊花种植较为集中的花卉基地和公园采集 2 0 8份病毒症状明显的菊花病标样 ,通过鉴别寄主反应、电镜观察、血清学反应以及RT -PCR扩增 ,鉴定出危害菊花的病毒病原有 :菊花B病毒 (CVB)、番茄不孕病毒(TAV)、马铃薯Y病毒 (PVY)、马铃薯X病毒 (PVX)、黄瓜花叶病毒 (CMV)和烟草花叶病毒 (TMV) ,其中CVB为侵染危害菊花的优势病毒 ,占 5 2 .5 % ,田间多数情况下是与TAV ,PVY ,PVX等病毒复合侵染。

A total of 208 samples of chrysanthemum showing symptom of virus diseases were collected in Kunming. The six kinds of viruses were identified on the basis of identification host reaction, virus particle physical properties, serology tests and RT-PCR identification results. They were chrysanthemum B virus (CVB), tomato aspermy virus (TAV), potato Y virus (PVY), potato X virus (PVX), cucumber mosaic virus (CMV), and tobacco mosaic virus (TMV) respectively. CVB was the most common virus on chrysanthemum in Kunming, and it got 52.5%, and it often occurred recombination infection with TAV, PVY, PVX.