摘要
以马立克氏病病毒 (MDV) 648株基因组DNA为模板 ,用PCR方法扩增 gI基因 ,将其插入到鸡痘病毒转移载体pFG1 1 75 1 ,通过脂质体方法转染鸡痘病毒 (FPV)感染的鸡胚成纤维细胞 (CEF) ,用蓝斑筛选方法纯化 4次 ,得到重组FPV ,用PCR方法证实 gI已插入到FPV基因组中 ,以间接免疫荧光试验鉴定重组FPV在CEF中表达了 gI基因 ,重组FPV命名为rFPVMDV648gI。
Marek′s disease virus (MDV) gI gene was amplified by PCR from MDV648 strain genome and inserted into a fowlpox virus (FPV) transfer plasmid pFG11751. The plasmid carrying gI gene was named pFGgI11751. The recombinant virus were screened by blue\|plague method for 4 times and identified by PCR. Chick embryo fibrobast (CEF) cells which had infected FPV were transfected with pFGgI11751. The expression of MDV gI gene in rFPV was confirmed by immunofluorence assay with specific monoclonal antibody. The recombinant FPV was named as rFPV\|MDV648gI.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
2002年第1期6-8,共3页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家 8 63高新技术发展项目 (863 10 10 5 0 3 0 2 )
