鸡胚PGCs迁移与性腺发育关系的研究

RELATIONSHIP BETWEEN PGCs MIGRATION AND GONAD DEVELOPMENT IN THE EARLY CHICKEN EMBRYO

采用连续切片法进行鸡胚胎显微观察 ,研究鸡原始生殖细胞 (PGCs)迁移特性及其与性腺发生、发育的关系。结果表明 :①在孵化 5 3～ 5 6h时鸡胚PGCs到达未来性腺形成区域 (体腔上皮 ) ,且在PGCs到达之前 ,这一区域已开始增厚 ;孵化 5 3～ 5 6h时 ,体腔上皮的脏壁层发现有PGCs,说明生殖嵴的发生亦在此期。②PGCs最终迁移至增厚的体腔上皮 ,部分体腔上皮的增厚区域趋向中肾 ,说明中肾和生殖嵴的发生均有体腔上皮增厚活动的参与。③孵化前 5d ,PGCs仍保持典型特性 ;孵化 7d后 ,PGCs细胞质中的糖原完全消失。④PGCs刚进入未来生殖腺形成区域时呈无序排列 ,孵化 6d时大多数从中肾侧向外移动 ,排列于未来性腺的上皮层 ,此时性腺已具有雌雄分化的特征。

The relationship between PGCs migration and gonad development of chick embryo which was incubated for 3 hours^7 days were studied. The results showed that when embryo was hatched for 53~56 h, the PGCs reached the epithelial tissue of body cavity where gonad would be formed. The epithelial tissue began thicker before the PGCs reached. At the 3.5~5th hatching day, the glycogen in the PGCs cytoplasm reduced gradually. On the 6th hatching day, the gonad of the embryo exhibited the feature of ovary, and the glycogen in the PGCs cytoplasm reduced further. On the 7th hatching day, the differentation of ovary or testis was obvoius and the glycogen in the PGCs cytoplasm vanished completely.