絮凝剂在青霉素酰化酶提取中的应用

Applications of flocculating agents in extraction of penicillin acylase

选用一种阳离子絮凝剂甲壳素对E .coli10 8发酵液进行处理 ,并改进渗透压提取青霉素酰化酶的方法 ,使酶活的提取率达到了 96 .4 % ,降低了能耗 ,酶的比活力达到 6 .4 9× 10 -6mol·min-1·mg-1。

Fermented solution from E.coli108 was processed with flocculating agents and Penicillin acylase was extracted by improved osmotic shock method. The total recovery was 96.4% and the specific activity of crude was 6.49×10 -6 mol·min -1 ·mg -1 .