SSR和ISSR分子标记及其在植物遗传育种研究中的应用

SSR marker, ISSR marker and their application to plant genetics and breeding

SSR(SimpleSequenceRepeat)和ISSR (Inter-SimpleSequenceRepeat)技术是在PCR基础上发展起来的两种DNA多态性检测技术 ,已开始应用于基因组研究的各个领域。概述了SSR、ISSR反应的原理、特点 ,总结了其在植物亲缘关系和遗传多态性研究、DNA指纹库的建立、遗传图谱的构建和基因定位及分子标记辅助育种等方面的应用 ,并肯定了SSR。

Simple sequence repeats (SSR) and Inter-simple sequence repeats (ISSR) are two kinds of DNA markers based on the polymerase chain reaction (PCR). They have been applied in many aspects of genome research. This paper has clarified the theories and characteristics of SSR as well as ISSR. The authers also summarized their applications in genetic polymorphisim and genetic relationship, establishment of DNA fingerprinting pool, construction of genetic map, gene localization and marker-aided selection. The two methods of DNA ecular marker open up broad prospects for the studies of plant genetics and breeding.