雷公藤醋酸乙酯提取物亚慢性中毒的实验病理学研究

An Experimental Pathologic Study of Subchronic Toxicityby Ethylacetate Extract of TripterygiumWilfcrdii Hook

将小剂量雷公藤醋酸乙酯提取物混入饲料喂饲纯种Wistar大鼠6个月,实验组大鼠病理检查见淋巴器官的淋巴小结、脾小结萎缩,淋巴细胞数目减少;雄性大鼠曲细精管上皮细胞变性、坏死、数目减少;酸性α-萘酚醋酸酯酶(ANAE)酶标检查示T/B淋巴细胞比值上升,说明B淋巴细胞对本品较T细胞敏感。

Subchronic toxicity was induced in rats by exposure to ethylacetate extract of Tripterygium Wilfordii Hook for six months. At the end of the experiment, pathologic changes in main organs were studied by light and electron microscopy. Acid-naphthyl acetate esterase (ANAE) technique was also used for identifying the ratio change between T and B lymphocyte in lymphoid organs. The number of lymphocytes decreased in lymphoid organs, especially in B lymphocyte distributing area. Obvious degeneration and necrosis were not observed in lymphoid organ, but the ratio of T/B lymphocytes increased. The spermatogenic cells of testis were degenerated, and decreased in number. In lowest dosage group, serious pathologic changes of spermatogenic cells were observed. There were, however, no pathologic changes in the heart, liver and kidneys etc.