热毒清抗矽尘毒性效应的细胞化学定量观察

A Quantitative Cytochemical Observation of Antitoxic Effect of Traditional Chinese Medicine "Re Du Qing" on Silica Particles

为进一步探讨中药热毒清抗矽尘毒性效应,对不同培养阶段的5组小鼠腹腔巨噬细胞用形态学和细胞化学方法,对巨噬细胞的形态及ACP活性,用显微镜及显微分光光度计进行了定性、定量动态观察。结果:吞噬矽尘后的SiO_2组巨噬细胞远较中药、P_(204)及联合用药组迅速死亡,崩解。细胞内ACF活性也大大低于其他组(P<0.01),但中药与P_(204)组间差异无显著性(P>0.05)。认为,热毒清有明显的抗矽尘毒性效应。

In order to further elucidate the antitoxic effect of traditional Chinese medicine 'Re Du Qing' on silica particles, we made an observation on the routine morphology of the cells with acid phosphatase (ACP) staining, and on the quantitative dynamic measures of ACP activities in various cultural stages of purifies peritoneal macrophages of mice with microscope and microscopectropho-tometer (Univar-MSPM) in five groups. The results showed that after phagocytosis of the silica particles, the macrophages in the SiO2 group died and broke down: much more rapidly than those in the 'Re Du Qing' and P204 groups, and the in-tracellular ACP activities in the SiO2 group were also much lower than those in other ones (P<0.01). However, there were no significant differences between ACP activites in the 'Re Du Qing' and P204 groups (P>0.05). From the above it may be suggested that the traditional Chinese medicine 'Re Du Qing' has significant antitoxic effect on silica particles.