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利用cDNA微阵列技术筛选和鉴定NYD-SP9基因 被引量：3

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摘要 运用cDNA微阵列杂交技术从人睾丸cDNA文库中筛选出人蛋白激酶基因,命名为NYD-SP9.NYD-SP9在成人睾丸中的表达水平比胚胎睾丸高10倍.Southern印迹杂交结果表明,这一基因高表达于睾丸,而低表达于卵巢.cDNA序列的蛋白质基序磷酸化位点分析也进一步提示,它有可能参与精子发生中的信号转导过程.由于这些磷酸化位点的位置彼此很接近,在这些位点之间可能存在相互作用以调控精子发生.该基因含有一个保守的穿膜功能结构域,提示经翻译后为一跨膜蛋白.以上实验结果表明,蛋白激酶基因NYD-SP9可能在男性生殖细胞分化过程中发挥一定作用.
作者 肖俊华 尹岚岚 李健民 周作民 朱虎 沙家豪 赵白鸽
机构地区 南京医科大学生殖医学江苏省重点实验室 国家计划生育委员会
出处 《科学通报》 EI CAS CSCD 北大核心 2002年第4期293-297,共5页 Chinese Science Bulletin
基金 本工作为国家重点基础研究发展规划资助项目(批准号:G1999055901)。
关键词 cDNA微阵列技术 筛选 鉴定 NYD-SP9基因 人睾丸 差异表达 精子发生
分类号 Q781 [生物学—分子生物学]
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参考文献27

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同被引文献64

  • 1何坤,高歌.DNA芯片技术简介[J].计算机教育,2006(9):19-23. 被引量：1
  • 2贾咏存,魏莎莉.人类Y染色体微缺失与精子生成障碍的研究进展[J].国际遗传学杂志,2006,29(4):273-276. 被引量：11
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  • 10Magarinos B, Toranzo A E, Barja J L. Existence of two geographically -linked clonal lineages in the bacterial fish pathogen[J].J Bacteriol.2000, 201 : 6 384-6 388.

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科学通报
2002年 第4期

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