石油降解细菌的表型特性和系统发育分析

Phenotypic characteristics and phylogenetic analysis of crude oil degrad-ing bacteria

从3种不同土壤中分离和纯化得到10个石油降解细菌菌株。分离菌株均为好氧菌、革兰氏阴性菌、不形成芽孢的杆菌。10个菌株均能利用中等链长的烷烃、柴油和原油作为碳源，而不能以单环芳烃和多环芳烃为碳源。根据其生理生化性状和 16S rDNA序列分析结果表明，分离菌株 EVA5，EVA6，EVA7，EVA8和 EVA9为假单胞菌（Pseudomonas spp. ），EVA10、EVA11、EVA12、EVA13和 EVA14为不动杆菌（Acinetobacter spp），均属于 Proteobac－teria的γ亚群。

Ten strains of crude oil degrading bacteria were isolated from three soil samples. The isolateswere Gram-negative, aerobic, non-spore-forming rods. All of the ten isolates were able to grow on n-al-kanes with medium length, diesel fuel and crude oil, but not polycyclic aromatic hydrocarbon. Based onphenotypic characteristics and 16S rDNA sequence analysis, ten isolates were identified as Pseudomonasspp. (strain EVA5, EVA6, EVA7, EVA8, EVA9 ) and Acinetobacter spp. (strain EVA10, EVA11,EVA12, EVA13, EVA14 ), respectively.