摘要
为了获得适合流式细胞仪检测的样品。通过碘化丙啶标记和流式细胞仪对食管癌细胞内DNA含量分布进行分析 ,在不同的时间内对血液中淋巴细胞内DNA含量的测定结果进行研究。结果显示 :结构完整的细胞DNA的荧光点图形成二倍体、四倍体的区域。组A各样品具有良好一致性 ,1～ 3天内差异小 ;组B各样品差异较大。因此细胞的完整性是DNA含量检测的基本条件。细胞经PI染色后若不能及时检测 ,可避光、4℃放置 1～ 3天仍可获得较理想的实验结果。
To develop a proper sample preparation method for flow cytometry. Using propidium iodide labeling and flow cytometry, we analyzed distributive image of DNA content in cell of esophageal carcinoma and compared DNA content of lymphocytes in blood during difference times. Dot plot image of DNA fluorescence could form region of diploid and tetraploid in integrated cell. All samples of group A had a good consistent and was little difference in three days, but that of group B had a great diversity. Cellular integrity is basic condition of measurement on DNA content. After propidium iodide labeling, if cell is not tested on time, it could be keeped away from beam and stored at 4℃. We can get good result in three days.
出处
《激光生物学报》
CAS
CSCD
2002年第2期122-125,共4页
Acta Laser Biology Sinica
关键词
流式细胞仪
检测
细胞DNA
样品制备
flow cytometry
DNA content
propidium iodide
sample preparation
