剥粒菠萝组织培养及快速繁殖的研究

Study on Tissue Culture and Macropropagation of Peeling-pineapple

剥粒菠萝是目前菠萝品种中最适于鲜食的良种之一。本研究以剥粒菠萝为材料 ,通过 1 0种培养基的诱导筛选、繁殖生根 ,从中选出最适宜剥粒菠萝组培快繁的一整套稳定、高效的生产程序 ,即 :芽诱导培养基为MS +6_BA 3mg/L(单位下同 ) +NAA 0 .1 ,增殖培养基为MS+6_BA 4+NAA 0 .1 ,生根培养基为 1 /2MS +6_BA 2 +NAA 0 .2 +活性炭 0 .1 %。该组培技术的成功 ,为规模化生产剥粒菠萝提供了快速繁殖种苗的技术保障 ,有利于促进南方地区名优水果的发展 ,有利于南方农业结构的产业化调整。

Peeling_pineapple (Anana comosus Merr. `Baoli') is one of the improved varieties of pineapples which can be eaten uncooked. The stem tips of peeling_pineapple seedlings were used as explants. After screening experiments from 10 culture media for bud induction and rooting, a stable high efficient procedure for macropropagation of peeling_pineapple was established. The proper bud induction medium was MS+6_BA 3mg/L (the unit is the same below)+NAA 0.1; the multiplication medium was MS+6_BA 4+NAA 0.1; the rooting medium was 1/2MS+6_BA 2+NAA 0.2+active carbon 0.1%. We offer a macropropagation method to the large scale production of peeling_pineapple plants.