肺炎衣原体的培养及对人脐带静脉内皮细胞的感染

Replication of Chlamydia Pneumoniae in HEP-2 Cells and Human Endothelial Cells

目的 研究肺炎衣原体的培养及其对人脐带静脉内皮细胞的感染 .方法 以人喉表皮癌(HEP- 2 )细胞培养肺炎衣原体并感染人脐带静脉内皮细胞 ,光镜及电镜下观察感染情况并计包涵体数 .结果 肺炎衣原体能在 HEP- 2细胞内培养并能感染人脐带静脉内皮细胞 ,感染后 72 h,包涵体数最多 ,96 h后仍可见肺炎衣原体在人脐带静脉内皮细胞内存活 .结论 肺炎衣原体能感染并存活于人脐带静脉内皮细胞 ,能导致动脉内膜感染 .

Objective To investigate whether Chlamydia pneumoniae (C.pn)could infect of human umbilical vein endothelial cells(HUVE cells). Methods After cultured in HEP-2 cells,C.pn was infected of HUVE cells,the inclusions were observed by ectromicroscoper.Results C.pn was able to grow and multiply in HEP-2 cells,infect and live in HUVE cells. Conclusion The ability of C.pn to grow in HUVE cells suggests a role for C.pn as a localpathogenetic factor in vascular inflammatory alterations, including atherogenesis.