PGP9.5和S-100蛋白在先天性巨结肠诊断中的应用

The Application of PGP 9.5 and S-100 Protein Immunohistochemical Method in the Diagnosis of HD

目的 探讨神经标志物蛋白基因产物 9.5 (protein gene product9.5 ,PGP9.5 )和 S- 10 0蛋白在先天性巨结肠 (Hirschsprung′s disease,HD)诊断中的作用 .方法 直肠粘膜活检标本 5例、手术切除标本 13例及正常对照 2例 ,行一抗为抗 PGP9.5及抗 S- 10 0蛋白的免疫组织化学检查(PAP法 ) .结果 所有活检及手术切除标本 PGP9.5标记粘膜下和 /或肌间神经丛内未见神经元 ,S- 10 0标记神经丛内无细胞状的“空白”区 ,但均可见神经纤维增粗且排列紊乱 .对照组 PGP9.5标记粘膜下和 /或肌间神经丛内见黄褐色的神经节细胞 ,而 S- 10 0标记神经丛内见细胞状的“空白”区 .结论 PGP 9.5和 S- 10 0蛋白免疫组织化学检查方法可用于诊断 HD.

Objective To investigate the application value of PGP 9.5 and S-100 protein immunohistochemistry in the diagnosis of hirschsprung′s disease.Methods 13 specimens,extracting from rectal biopsy and definitive operation, were performed with immunohistochemical approach using antibodies that react to PGP 9.5 and S-100 protein respectively. The results were compared with that in control (n=2). Results In HD group, PGP 9.5 positive neurons were not found in the myenteric and the submucous plexus. The 'cell-like blank' areas were not detected in the specimens treated by S-100 protein. Both PGP 9.5 and S-100 protein positive fibres were increased in number and size, forming irregular nerve bundles. In control group, both PGP 9.5 positive neurons and the 'cell-like blank' areas appeared in the myenteric and submucous plexus. The positive fibres were abnormal.Conclusions PGP 9.5 and S-100 protein immunohistochemical method can be used for the diagnosis of HD.