宽叶缬草对高胆固醇血症大鼠肾脏保护作用的探讨

Protective Effect of Valeriana Officinalis Var Latifolia on Lipid Nephropathy in Hypercholesterolemic Rats

目的 :探讨宽叶缬草在脂质肾损害中的保护作用和可能机制。方法 :用含 4 %胆固醇和 1%胆酸钠的高脂饲料饲喂大鼠建立高脂模型 ,观察宽叶缬草对大鼠血脂、尿蛋白和肾功能的影响 ,以及对肾小球系膜细胞增殖、细胞外基质增生、α -SMA表达、Ⅳ型胶原表达的影响。结果 :宽叶缬草有降低总胆固醇、低密度脂蛋白和尿蛋白的作用 (P <0 .0 1) ,肾病理和免疫组化证实宽叶缬草在降脂和降低尿蛋白同时 ,能减轻肾小球系膜病变和细胞外基质产生 ,降低肾小球α -SMA和Ⅳ型胶原表达。结论

Objective:To evaluate the protective effect of Valeriana officinalis var latifolia(VOL) on the kidneys of hypercholesterolemic rats and study its possible mechanisms.Methods:Dietary-induced hypercholesterolemia was induced in male Wistar rats by giving 4% cholesterol and 1% cholic acid diet lasting for 16 weeks.Changes of serum lipid,urinary protein excretion,renal function,Mesangial cell index and Mesangial matrix index were assessed;Moreover, immunohistochemical stain for α-smooth muscle actin and type Ⅳ collagen were performed.Results:VOL not only could reduce the serum levels of total cholesterol and low density lipoprotein,but also could reduce urinary protein excretion.Light microscopic and immunohistochemical stain revealed that lowing serum lipids and urinary protein,Mesangial cell index and Mesangial matrix index were significantly reduced in the same time,accompanied with decreased expression of α-SMA and type Ⅳ collagen.Conclusion:It is concluded that VOL has the effect of regulating lipid metabolism,attenuating renal damage .The inhibition of glomerular mesangial cells undergoing phenotypic change might be the mechanism of VOL on renal protection.