摘要
以PCR方法扩增得到一个猪α干扰素的基因 ,克隆入T载体后测序 ,发现其序列与猪α1干扰素基因成熟肽编码区序列有 14个碱基差别 ,同源性达 97%。其中第 12 4位变异为T ,导致形成TAG终止密码子。综合推断其为猪α干扰素假基因。该基因序列的GENEBANK登录号为AF35 0 4 2 5。
A pseudogene of porcine alpha interferon with a te rmination codon TAG in its mid was isolated from swine genomic DNA by PCR method . The cloned ΨPoIFNα gene was 570 nucleotides long , with 14 bases different from the reported coding gene sequence of PoIFNα 1. The Genebank registration number of the sequence was AF350425.
出处
《上海农业学报》
CSCD
2002年第2期72-75,共4页
Acta Agriculturae Shanghai
基金
上海市农业科学院重点学科青年科技骨干培养基金
