猪瘟抗体监测及免疫效果分析

Detection of Serum Antibody Against Hog Cholera in Pigs

对 9个规模化养猪场 15 18份血清进行了猪瘟免疫抗体检测。 7日龄、2 0日龄和 4 0日龄仔猪无免疫保护力 (血凝抗体效价低于 1∶16 ,下同 )的比例分别为 0、4 .8%和 19.7% ;而抗体效价≥ 1∶12 8的比例分别为 87.8%、74 .6 %和 35 .2 %。乳前免疫后 2 0、30、4 0、5 0和 6 0日龄无免疫保护力仔猪的比例分别为 18.4 %、8.4 %、9.8%、10 .0 %和 15 .4 % ,而抗体效价≥ 1∶8的比例分别由 4 0日龄的 4 1.4 %降至 5 0日龄的 15 .0 %和 6 0日龄的 3.8%。所测 398份 2 5日龄仔猪母源抗体效价均≥1 16 ,但抗体效价≥ 1 12 8的比例由 2 0日龄的 92 .8%降至 2 5日龄的 6 2 .5 %。提示猪群中存在不同比例的免疫不合格和低抗体水平的猪 。

The serum samples from 9 intensive pig farms were tested for antibody against hog cholera. Among the 207 serum samples, 0%, 4.8%, and 19.7% checked sera from 7 day old, 20 day old, and 40 day old piglets had antibody titer less than 116 which was regarded as threshold of protective level. The percentage of sera with antibody titer 1∶128 or more were 87.8%, 74.6%, and 35.2% for 7 day old, 20 day old, and 40 day old piglets respectively. After immunizing newborn piglets without sucking, there were 18.4% , 8.4%, 9.8 %, 10.0%, and 15.4% sera from 20 day old, 30 day old, 40 day old, 50 day old, and 60 day old piglets respectively with antibody below the threshold of protective level. The percentage of sera with antibody titer 1128 or more decreased from 41.4% for 40 day old piglets to 15.0% for 50 day old piglets, and 3.8% for 60 day old piglets respectively. In measuring maternal antibody of piglet, all 398 sera from 25 day old piglets had antibody titer above 1∶16, but the percentage of sera with antibody titer 1∶128 or more decreased from 92.8% for 20 day old piglets to 62.5% for 25 day old piglets.