双梯度超薄胶PAGE分离DNA及其银染与回收

DNA Fragments Separating by Double-gradient Super-thin PAGE and Recovering from Silver-stained Gels

双浓度梯度超薄胶 PAGE分离 DDRT-PCR扩增产物表明 ,在 3 0 cm长的凝胶板上能清晰分辨长度仅差 1 bp的 DNA,清楚的显示出 1 0 0多条分离谱带 ,并可直接从银染 PAGE胶中回收 DNA谱带。简便节省 。

DDRT PCR amplification products were separated in double gradient super thin PAGE. The results showed that single base pair(bp)difference between two DNAs was clearly detected in 30 cm long gels, and over 100 bands were completely separated by the double gradient PAGE. On the other hand the objective bands could be directly recovered from silver stained PAGE gels. The method issimply, economical,and makes it possible availably to analyes gene xpression.