摘要
吸胀而未萌发的豇豆 (VignaunguiculataL .)种子检测不到多胺氧化酶 (PAO)活性 ,直至萌发开始 (吸胀后 36h)才在胚芽、子叶和胚轴测到PAO活性 ,此后各部分的PAO活性以不同速度增加 ,而胚根一直没能检测到PAO活性。蛋白质合成抑制剂环己酰亚胺 (CHM)吸胀处理可显著抑制萌发种子胚芽和子叶的PAO活性 ,同时强烈抑制种子的萌发 ;核酸合成抑制剂放线菌素D处理对豇豆胚芽和子叶的PAO活性以及种子萌发的影响则远不及CHM处理。豇豆种子在黑暗条件下萌发 ,胚芽和子叶的PAO活性都明显高于光下。适宜浓度的腐胺和CuCl2 吸胀处理也可提高胚芽和子叶的PAO活性。
No PAO activity was detected in ungerminated cowpea( Vigna unguiculata L.)seeds(form 0 to 24h after imbibition),but it could be detected in the plumule,cotyledons and embryonic axis of the germinating seeds whose radicles were just visible(36h after imbibition).The PAO activities in these three parts increased at different rates during germination,while no PAO could be detected in radicle at all times.Soaking with Act-D only inhibited the seed germination by 27.6%,but CHM could inhibit the germination by 90.6%.Moreover,the inhibitions of the PAO activity in the plumule and cotyledons by Act-D were just 29.1% and 33.3%,and those by CHM were up to 87.2% and 85.4% respectively.Light had a remarkable inhibitory effect on the PAO activity in plumule and cotyledons.Soaking with putrecine(0.1 and 0.5mmol/L)or CuCl_2(10μmol/L)for 6h could enhance the activity of PAO.
出处
《园艺学报》
CAS
CSCD
北大核心
2002年第2期153-157,共5页
Acta Horticulturae Sinica
基金
广东省自然科学基金资助项目 ( 0 0 14 2 5 )
关键词
豇豆
种子萌发过程
多胺氧化酶活性
变化
CU^2+
Cowpea( Vigna unguiculata L.)
Polyamine
Polyamine oxidase
Seed germination
Cu 2+
