梨自交不亲和强度不同品种花柱S糖蛋白含量的差异

Differences of S-glycoprotein Content in the Styles among Pear Cultivars Differing in Self-incompatible Strength

提取梨 (Pyrus)自交不亲和强度不同品种花柱可溶性蛋白质 ,应用等电聚焦电泳法在凝胶板上分离出S糖蛋白 ,用图像解析法进行定量。结果表明 ,花柱总S糖蛋白含量因品种而异 ,自交不亲和强、弱及自交亲和的品种每 1μg花柱可溶性蛋白中含总S糖蛋白分别为≥ 1.3ng、 0 .7～ 0 .9ng、≤ 0 .7ng。不同的S基因所表达的S糖蛋白量差异显著 ,即使是同一S基因所对应的S糖蛋白量也因品种而异。

Stylar soluble proteins were extracted from the whole style of pear cultivars differing in self-incompatibility (SI) strength and were separated by isoelectricfocusing polyacrylamide gel electrphoresis (IEF-PAGE) and each S-glycoprotein content was quantified by an image analyzer. Total S-glycoprotein (sum of two allelic-linked S-glycoproteins) content differed among cultivars. The cultivars which were strong SI, weak SI and sel-compatibility contained ≥1.3 ng, 0.7-0.9ng, and ≤0.7ng of S-glycoprotein per μg of stylar soluble protein, respectively. S -allele linked glycoprotein content differed greatly in various S -genotypes. Furthermore, the S-glycoprotein content was even different in cultivars with the same S genotype.