猪瘟三种快速诊断方法的比较

Comparison of Three Diagnostic Methods for Detection of Classical Swine Fever

本试验运用电镜负染、荧光抗体染色、间接－ＥＬＩＳＡ三种方法对新疆南疆地区收集的７份猪瘟ＲＴ－ＰＣＲ阳性病料进行了检测，发现：电镜负染法检出阳性病料２份，荧光抗体染色法检出阳性病料６份、间接－ＥＬＩＳＡ法检出阳性病料６份。结果表明荧光抗体染色、间接－ＥＬＩＳＡ都有很好的敏感性和特异性，其中间接－ＥＬＩＳＡ法可作为基层对猪症进行快速诊断的首选方法。

Seven clinically pathological samples of classical swine fever were deteched with tha different methods: electron microscopy, fluorescent staining, indirect ELISA respectively.The results showed that fluorescent staining and indirect ELISA had the same sensitivity and specificity,and were more sensitive than EM.The indirect ELISA was considered to be the best method for the diagnosis of classical swine fever.