PEG6000修饰物混合吐温80-硫酸铵盐的液-固萃取体系分离纯化血红蛋白

Separation and Purification of Hemoglobin by Using Liquid-Solid Extraction System of Cu(Ⅱ)IDAPEG6000-Tween 80-(NH_4)_2SO_4

采用聚乙二醇 6 0 0 0 (PEG6 0 0 0 )修饰物混合吐温 80 硫酸铵盐的液 -固萃取体系对血红蛋白分离纯化进行研究 ,考察了修饰物、盐、吐温 80浓度以及体系酸度、温度等因素对血红蛋白分离纯化的影响 ,用该体系直接从三种血液中分离纯化血红蛋白 ,操作简便快速 ,萃取容量大 ,一次萃取固相收得率 99%以上 ,一次反萃取率达 75 % ,一步纯化倍数达 2 0倍 ,用多种手段验证了所获猪血红蛋白 ,纯度达 99% .

The method of separation and purification of hemoglobin by using liguid-solid extraction system of Cu(Ⅱ)-IDAPEG 6000-tween 80- (NH 4) 2SO 4 was studied. The influences of conditions such as the amount of Cu(Ⅱ)~IDAPEG 6000, concentrations of Tween 80 and salt, acidity, temperature, etc., were studied. It was simple and rapid to extract pig hemoglobin, human hemoglobin and bovine hemoglobin from blood using this extraction systems, with high extraction capacity and extraction rate. The one-step solid phase extraction rate was more than 99%, and the one-step reextraction rate was 75%. Hemoglobin purification fold was about 20.00. The purity of pig hemoplobin (PHb) in the sample reached 99%.