支原体肺炎患儿活性氧与内皮细胞变化的相关性研究

A Study on the Relationship between the Active Oxygen and Endotheliocyte in Children with Mycoplasma Pneumonia

目的:探讨小儿支原体(MP)肺炎时活性氧致内皮细胞损伤的机制。方法:应用Hladovec法、硝酸还原酶法和放射免疫法。检测46例MP肺炎患儿及25例正常健康儿血CEC数量、NO及TXB_2水平。结果:MP肺炎患儿急性期血CEC、NO及TXB_2均明显高于对照组(P<0.01),恢复期均明显降低,但CEC与NO仍高于正常对照组(P<0.01)。NO、TXB_2水平与CEC数量呈高度正相关。结论:MP肺炎患儿存在肺血管内皮细胞(VEC)损伤,NO、TXB_2可能对VEC损伤起重要作用。

Objective:To study the damage mechanism of active oxygen to endotheliocytes in children with mycoplasma (MP) pneumonia. Methods: The levels of CEC, NO and TXB2 in serum in 46 cases of children with MP pneumonia and 25 normal subjects were measured by use of Hladovec's method,carbonate reductase assay and radioimmunoassay.Results:The levels of CEC,NO and TXB2 in MP pneumonia of children were higher than that in the controls group ( P < 0.01) ,and decreased in their recovery phase, but CEC and NO levels were still higher than that in the controls group (P < 0.01) .Serum NO.TXB2 level positively correlated with CEC level.Conclusions:There may be injury of pulmonary vascular endothelial cell (VEC) . NO and TXB2 may play an important role in the injury of VEC.