复方醋酸氟轻松软膏含量测定方法的改进

The improve of the method for determination compound fluocinonide ointment

目的 :改进复方醋酸氟轻松软膏含量测定方法。方法 :采用RP -HPLC法 ,提取过滤后加内标。ODS柱 (2 5 0mm× 4 6mm ,5 μm) ,以甲醇 水 乙醚 (6 2 38 2 ,v v v)为流动相 ,检测波长为 2 4 0nm ,流速为 1 0ml·min-1。结果 :改进后的方法精密度及准确度均较满意。结论 :改进后的方法解决了原方法存在的问题 ,对软膏剂的含量测定具有普遍意义。

OBJECTIVE:To improve the method for determination compound fluocinonide ointment.METHODS:A method of RP HPLC be used, plus the internal standard after extraction and filter.ODS column (250mm×4 6mm, 5μm) was selected and the mobile phase was consisted of methanol water ether(62382,v/v/v) at flow rate of 1 0ml·min -1 .The detection wavelength was 240nm.RESULTS:The precision and the accuracy of improved method are good.CONCLUSION:The problem of the old method was solved with this improved method and have a universal significance for the determination of ointment preparation.