RP-HPLC法测定盐酸恩丹西酮氯化钠注射液的含量

Determination of ondansetron hydrochloride in natrium chloride infusion by a reversed-phase high performance liquid chromatography

目的 :建立反相高效液相色谱法测定盐酸恩丹西酮氯化钠注射液含量的方法。方法 :采用ODS反相柱 ,流动相为甲醇 - 0 0 2M磷酸二氢钾 (5 8 4 2 ,pH =6 0 ) ,检测波长为 30 8nm。结果 :盐酸昂丹司琼在辅料中的回收率为 10 0 14 % ,日内与日间精密度分别为 0 4 1%与 0 5 4%。结论 :本法准确、快捷 ,适用于测定盐酸恩丹西酮氯化钠注射液的含量。

OBJECTIVE:To establish a new method to determin ondansetron in a natrium chloride infusion.METHLDS:A reversed phase high performance liquid chromatography was employed. The mobile phase was composed of methanol and 0 02M KH 2PO 4 solution (5842, pH=6 0).ODS was the stable phase.RESULTS:Recovery was 100 14%. Between day and Within day precision was 0 41% and 0 54% respectively.CONCLUSION:The method was rapid and correct. It is suitable for the determination of ondansetron in natrium chloride infusion.