脾切除对单核巨噬细胞系统功能的影响

Effect of splenectomy on mononuclear phagocyte system function in presence and absence of sepsis

目的研究大鼠脾脏切除后在伴或不伴脓毒血症状态下单核巨噬细胞系统(MPS)功能的变化,探讨脾脏切除后感染发生的机理。方法用99mTc-植酸钠测定Wistar大鼠脾脏切除后在伴或不伴脓毒血症情况下MPS的功能,同时观察血浆纤维连结蛋白(PFn)和透明质酸(HA)的变化。结果脾切除后,尤其合并感染时,血中清除半衰期(T1/2)延长,吞噬指数K值、肝脏吞噬功能降低,PFn减少,HA浓度升高。结论PFn相对减少、HA增加参与MPS功能抑制及感染的发生。脾脏切除后不仅失去了一个MPS器官,还可以通过细胞和体液因子抑制MPS功能。

Objective To investigate the effect of splenectomy on mononuclear phagocyte system (MPS) function in presence and absence of sepsis. To study the mechanism of postsplenectomy infection. Methods The effect of splenectomy on MPS function was investigated by measurement of 99mTc-phytate in the presence or absence of sepsis in rats, and plasma fibronectin (PFn) and hyaluronic acid (HA) concentrations were measured. Results Following splenectomy, particularly in the presence of sepsis, the intravascular clearance (T1/2) delayed and HA increased, phagocytic index(K) and hepatic uptake and PFn decreased. Conclusion Splenectomy may not only have deleterious effects in terms of the host defence system, but may also cause MPS depression, especially in the presence of sepsis. There is an importance role of HA in the pathophysiology of postsplenectomy sepsis possibly.