25s rRNA基因部分序列比较在香菇栽培菌种鉴定上的应用

Identification of Seven Lentinula edodes Strains Based on Partial Sequence Analysis of 25s rRNA Gene

对目前中国 7个主要香菇栽培品种 :苏香、2 6、939、135、9311、申 10和 74 0 2的 2 5srRNA基因中的D1/D2片段进行了DNA序列测定 ,根据DNA序列测定结果对它们之间的同源性进行了分析。结果表明 ,所测定的 7个香菇栽培品种之间的亲缘关系非常近 ,同源性很高。通过聚类分析 ,根据香菇之间的亲缘关系 ,可以将这 7个品种分为 2类 ,即苏香、939和 9311为一类 ,2 6、申 10、135和 74 0 2为一类。

Seven main cultivation strains of Lentinula edodes widely used in China, Shuxiang, 26, 939, 135, 9311, shen10 and 7402, were analyzed in this study. The D1/D2 region of 25s rRNA gene was amplified by PCR and sequenced. Genetic similarities among these strains were analyzed by the results of D1/D2 sequencing. And the results indicated that their genetic relationship was very close. Based on the cluster analysis results of genetic relationship with Clustal method in PCGENE software, they were divided into two groups, one was Shuxiang, 939 and 9311; the other was 26, Shen 10, 135 and 7402. And their phylogenetic tree was established too.