SeGLP-1的分离纯化及硒活性结构分析

Separation, Purification and Analysis of Selenium Activity Structure of SeGLP-1

灵芝 (Ganodermalucidum)加硒深层培养后的菌丝 (含Se 16 0 0 μg/ g)分别经水及碱水提取、醇析、透析、Sevage法脱蛋白、DEAE cellulose柱层析纯化 ,得SeGLP 1。经SephadexG 10 0、聚丙烯酰胺凝胶电泳和紫外光谱分析鉴定 ,SeGLP 1为均一级分。GC与TLC分析表明 ,SeGLP 1含Gal、Glu、Xyl、Rha、Man ,其单糖摩尔比为Gal∶Glu∶Xyl∶Rha∶Man =0 .2 3∶1.0 0∶0 .72∶ 0 .2 5∶ 0 .0 9。SeGLP 1硒含量 16 42 μg/g。SeGLP 1中Se活性中心的结构可能为O =Se =O ,即Se取代了灵芝多糖GLP 1中 -OCH3结构上的CH3。

The polysaccharide containing selenium in Ganoderma lucidum (SeGLP 1) was isolated through water, lye and alcohol extraction ,dialysis, deproteining with Sevage method and purifying by chromatography of DEAE cellulose column. And SeGLP 1 was identified to be a homogeneous fraction with Sephadex G 100 and polyacrylamide gel electrophoresis and UV spectrum analysis, while Gal, Glu, Xyl, Rha, Man were determined to be components of SeGLP 1 by GC and TLC. The quantitative analysis of SeGLP 1 showed that the molar ratio of Gal∶Glu∶Xyl∶Rha∶Man=0.23∶1.00∶0.72∶0.25∶0.09, and that SeGLP 1 contained 1642μg/g selenium. And the results from IR, 77 Se NMR, 13 C NMR and Laser Raman spectra showed that the structure of activity center of selenium of SeGLP 1 might be O=Se=O,and CH 3 in -OCH 3 group of GLP 1 was substituted by selenium forming this structure.