猪细小病毒和猪伪狂犬病毒复合PCR检测方法的建立

Establishment of the Multiple PCR-based Diagnosis of Porcine Parvovirus and Porcine Pseudorabies

在已经建立的PPV和PRV的单项PCR诊断方法的基础上,通过对扩增条件的筛选,成功地建立了 PPV和PRV的复合PCR诊断方法,并应用于临床。利用一次PCR反应,即可同时扩增PPV的445 bp和PRV 217 bp的特异性片段。该方法的建立对临床上进行这2种疾病的鉴别诊断和混合感染的检测都具有重要意义。

On the basis of single PCR of PPV and PRV, multiple PCR-based diagnosis method was studied. The results showed that two specific fragments of 445 bp for PPV and 217 bp for PRV could be amplified in a single PCR. This method could differentiate PRV infection, PPV infection and mixed infection from the reproductive failure.