牛心朴和苦楝果提取物对桑天牛产卵及卵孵化的抑制作用

INHIBITING EFFECTS OF THE CYNANCHUM KOMALOVII TOTAL ALKALOIDS AND MELIA AZEDARACH FRUIT EXTRACTS ON OVIPOSITION AND EGG HATCH IN APRIONA GERMARI (COLEOPTERA, CERAMBACIDAE)

使用一定浓度的牛心朴总生物碱苦楝果提取物处理的构树枝条作为天牛成虫的食物 ,比较了其对桑天牛产卵量及卵孵化率的影响。在室温下 ,将成对的天牛放在盖有铁纱网的直径 2 5cm高 2 5cm的塑料桶中进行试验。在 15d试验中 ,用 30 0 μg·mL- 1 的牛心朴总生物碱、10 0 0 μg·mL- 1 的苦楝果提取物处理枝条的两处理组的各 7对天牛其日平均产卵量分别为 3 5 3± 4 18个和 6 73± 3 82个 ,而对照组 7对天牛的日均产卵量为 13 73± 2 90个。经t—检验处理组与对照组之间在日均产卵量方面差异极为显著。 30 0 μg·mL- 1 的牛心朴总生物碱和 10 0 0 μg·mL- 1 的苦楝果提取物对桑天牛卵孵化有显著影响。其影响表现在两方面 :(1)孵化率降低。成虫取食 30 0 μg·mL- 1 牛心朴总生物碱和 10 0 0 μg·mL- 1 苦楝果提取物处理的构树枝条后产的卵最终孵化率分别为 77 36 %、87 12 %。而对照组的最终卵孵化率为 99 5 %。 (2 )其孵化期延长。牛心朴处理组的最终孵化期为 17天、苦楝果处理组的最终孵化期为 15d、而对照组的最终孵化期为 11d。

The branches of Broussonetia papyrifera were treated with Cynancum komalovii total alkaloids and Melia azedarach fruit extracts as the food of the beetle ( Apriona germari Hope), the effects of C.komalovii total alkaloids and M.azedarach fruit extracts on their oviposition and egg hatch were investigated and compared. The testing paired beetles were put into the plastic containers with diameter of 25?cm and 25?cm in height and covered with iron screen. The experiments were carried in laboratory at the room temperature. During 15?d testing period the average egg numbers laid per d of the treated seven pairs of the beetles in the two treated groups, which were fed on branches treated by 300?μg·mL -1 C.komalovii total alkaloids and 100?μg·mL -1 M.azedarach fruit extracts were 3.53±4.18 and 6.73±3.82 respectively and in the control group it was 13.73±2.90. t\|test showed that there were significant differences between the treated and control groups in average egg number per d. The Cynancum komalovii total alkaloids and the Melia azedarach fruit extracts affected egg hatching in A.germari, which were shown in two aspects:(1) The egg hatch rate was lowered. the final egg hatch rate in the group of 300?μg·mL -1 C.komalovii total alkaloids and in the group of 1000?μg·mL -1 M.azedarach fruit extracts were 77.36%,87.12% respectively, and that in control group was 99.5%. (2) The time period needed for egg hatch was increased. In the group of 300?μg·mL -1 C.komalovii total alkaloids, the time for egg hatch was 17 d , in the group of 1000?μg·mL -1 M.azedarach fruit extracts it was 15?d and that was 11?d in the control group.In brief, in the experiment it was shown that oviposition and egg hatch were significantly inhibited for the adults of Apriona germari, which had been fed with C.komalovii total alkaloids and M. azedarach fruit extracts. To develop new ways of behavior Manipulation for Insect Pest Management with the extracts of C.komalovii and M.azedarach were discussed with resources of the two plant species in China in the paper.