白背叶根在肝纤维化动物模型中抗氧化作用的实验研究

The Study on the Anti-oxidation Effect of Root of Mallotus apelta in the Rat Model of Liver Fibrosis

目的:通过大鼠肝纤维化动物模型,研究白背叶根防治肝纤维化的作用以及白背叶根抗氧化能力的研究。方法:采用40％CCl_4花生油溶液制备大鼠肝纤维化模型,观察白背叶根对肝纤维化大鼠血清和肝组织中多种指标的影响。观察的指标包括:丙氨酸转氨酶(ALT)、天门氨酸转氨酶(AST)、MDA、NO和肝组织中羟脯氨酸的含量以及肝组织损伤程度(病理切片观察)。结果:白背叶根能显著降低大鼠血清中多项指标的水平,并能减轻肝脏胶原纤维增生程度。结论:白背叶根具有较好的抗肝纤维化作用和抗氧化能力。抗氧化作用可能是白背叶根阻断肝纤维化形成的机理之一。

Objective: To investigate the effect of root of Mallotus apelta in treatment of liver fibrosis and the effect of anti-oxidation in the rat model of liver fibrosis. Methods: A rat model of liver fibrosis was induced with 40% CCl4 in peanut oil. A series of items were detected, which includ ALT, AST, MDA, NO and hydroxyproline in the blood or liver trssues. Results: The level of ALT, MDA, NO in serum were significantly decreased, the level of hydroxyproline was declined too, while the formation of liver fibrosis was successfully ameliorated. Conclusion: The root of Mallotus apelta could retain the progression of liver fibrosis, having a capacity of anti-oxidation. And the latter might be the reason of the former function.