漏芦中蜕皮甾酮提取方法研究

Extraction of Ecdysterone from Stemmacaniha uniflora

目的:优选漏芦中蜕皮甾酮的最佳提取工艺条件。方法:以蜕皮甾酮含量及固体物含量为指标,比较三种不同提取方法:不同溶剂回流法,超声提取法,冷浸法。结果:三种溶剂以甲醇提取蜕皮甾酮最完全,水其次,乙醇受浓度影响很大;超声提取所需时间短,提取基本完全;冷浸法所需时间长,提取不完全。结论:甲醇热提取法效果最好。

Objective: To optimize the conditions for the extraction of ecdysterone from Stemmacantha uniflora.Methods: Conditions for the extraction were studied by three differrent methods as guided by the content of ecdysterone and solid material that present in the extract. Results: Methanol was the best solvent, water the second, ethanol was greatly effected by concentration. Ultrasonic extraction had a good efficiency with short time. The time of cool extraction was too long, its efficiency was inferior to the other methods. Conclusion: The best methods for the extraction of ecdysterone from Stemmacantha uniflora was methanol reflux extraction.