抗大肠杆菌免疫卵黄球蛋白对大鼠放射性肠炎的防护作用

The prophylactic and protective effects of egg yolk immunoglobulin against Escherichia coli on radiation-induced enteritis in rats

目的 观察抗大肠杆菌卵黄免疫球蛋白 (IgY)对大鼠放射性肠炎的防护作用。方法 将 30只大鼠随机分为正常对照组 (A组 )、照射对照组 (B组 )和IgY保护组 (C组 )。大鼠全腹照射10 0 0cGy。C组自照射前 1d开始灌服IgY。 4d后处死大鼠 ,观察肠道细菌移位情况、血中内毒素水平及小肠粘膜病理形态学改变。结果 A组无细菌移位 ,B组细菌移位最为明显 (96 7% ) ,C组细菌移位 (13 3% )远不及B组明显 ;A组内毒素含量极低 (0 0 0 1EU ml) ,B组内毒素含量明显升高 (0 82 9EU ml) ,C组内毒素含量 (0 2 4 9EU ml)明显低于B组 ;A组肠粘膜正常 ,B组肠粘膜绒毛水肿 ,炎性细胞浸润 ,部分上皮细胞脱落 ,C组绒毛轻度水肿 ,未见明显细胞脱落。结论 全腹照射能明显损伤小肠粘膜 ,引起细菌移位和内毒素血症 ,抗大肠杆菌IgY能明显减轻射线对小肠粘膜的损伤。

Objective\ To observe the prophylactic and protective effects of egg yolk immunoglobulin (IgY)against Escherichia coli on radiation\|induced enteritis in rats. Methods\ Thirty rats were randomly divided into three groups:normal control group (group A),radiation control group (group B) and IgY treatment group (group C).The rats of groups B and C were subjected to whole abdominal irradiation of 1 000 cGy.Feeding IgY began since the day before irradiation in group C.Four days later,all the rats were killed and the intestinal bacteria translocation,the concentration of endotoxin in blood,and pathological changes of intestinal mucosa were measured or observed. Results\ Bacteria translocation was not found in group A,but evident in group B(96 7%),and much lighter in group C(13 3%) than in group B.The concentration of endotoxin in blood was very low in group A (0 001 EU/ml) and very high in group B (0 829 EU/ml),but it was much lower in group C(0 249 EU/ml) than in group B.The villus edema,infiltration of inflammatory cells in mucosa and epithelial desquamation were found in group B,but these pathological changes were much milder in group C and not found at all in group A. Conclusion\ Whole abdomen irradiation will evidently cause enteritis in rats,and followed by bacteria translocation and endotoxinemia;IgY against Escherichia coli can diminish these changes.\;