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抗人膀胱癌抗独特型抗体佐剂疫苗的动物实验 被引量:3

The animal study of immunotherapy of bladder carcinoma with anti-idiotypic antibody
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摘要 目的 :研究抗独特型抗体佐剂疫苗 (Ab2 +SAF)对膀胱癌的免疫治疗作用 ,为临床实验提供动物实验依据。方法 :用抗人膀胱癌Ab2 +SAF免疫BALB/C小鼠为实验组 ;对照组用生理盐水。免疫 3次后 ,于末次免疫后 1周 ,将新鲜人膀胱移行细胞癌组织移植于小鼠肾包膜下 ,分别于移植后第 2、4、6、8和 10天处死小鼠 ,取血清进行抗抗独特型抗体 (Ab3 )分析。移植瘤行组织学检查 ,观察宿主淋巴细胞浸润情况及瘤细胞可见率。结果 :实验组小鼠肾包膜下人膀胱癌细胞很快受到排斥 ,在移植后第 6天 ,淋巴细胞浸润即达高峰 ,而对照组在第10天才达高峰 ;瘤细胞可见率 ,在移植后第 6天即明显降低 ,而对照组呈逐渐下降。实验组Ab3 为阳性 ,而对照组为假阳性或阴性。结论 :Ab2 作为抗原免疫小鼠后 ,能够排斥瘤细胞的生长。 Purpose:To study the role of anti-idiotypic antibody in immunotherapy of bladder carcinoma and to investigate the effect of anti-idiotypic antibody (Ab 2) on bladder carcinoma in vivo.Method:BALB/C mice were immunized every week for 3 times with Ab 2 of bladder carcinoma which was connected with new type adjuvant-SAF.The control team were injected with salt water.Fresh bladder tumor cells were transplanted to the subrenal capsule of immunized mice after the last injection.Then the mice were executed separately on the 2 nd,4 th,6 th ,8 th,and 10 th days after the transplantation operations.Anti- Ab 2 (Ab 3) in sera was detected by using inhibition assay of enzyme-linked immunospecific assay.Tumor infilitration lymphocytes and tumor cells from the tumor tissue of mice were observed microscopically.Result:The sera levels of Ab 3in Ab 2immunized mice were higher than those of the controls. Tumor infilitration lymphocytes in the former were found to reach their highest peak on the 6 th day,where as the highest peak for the controls was on the 8~10 th days;tumor cell viability rate in the former decreased rapidly after 6 days,but in the controls decreased only gradually.Conclusion:Ab 2could prevent grow of tumor after mice were immunized with Ab 2.
出处 《临床泌尿外科杂志》 2002年第5期233-234,257,共2页 Journal of Clinical Urology
基金 卫生部科学研究基金资助项目 (No .98 1 2 0 6 )
关键词 抗独特型抗体 膀胱肿瘤 免疫治疗 佐剂疫苗 动物实验 膀胱癌 Anti-idiotypic antibody Bladder carcinoma Immunotherapy
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