明胶酶A基因高表达与胃癌浸润转移的关系

Effect of the Enhanced Expression of Gelatinase A Gene on the Invasion and Metastasis of Human Gastric Carcinoma

目的 研究明胶酶A基因表达对胃癌浸润转移的影响。方法 采用原位杂交技术检测 3 6例胃癌组织明胶酶AmRNA表达。结果 正常胃组织、癌旁和胃癌组织明胶酶AmRNA表达阳性率分别是 8.3 %、3 5 .7%和 83 .3 % ,三者间差异均有显著性意义 (P<0 .0 1)。有浆膜浸润和淋巴结转移的胃癌 ,其表达阳性率分别为 93 .1%和 90 .6% ,无浆膜浸润和淋巴结转移的胃癌其表达阳性率分别为 42 .9%和 2 5 .0 % ,差异均有显著性意义 (P<0 .0 1)。明胶酶AmRNA表达定位于肿瘤浸润边缘的基质成分 ,以巨噬细胞为主。结论 明胶酶AmRNA高表达可促进胃癌的浸润和转移 。

Objective To investigate the effect of mRNA expression of gelatinase A on the invasion and metastasis of human gastric carcinoma (HGC). Methods Thirty six cases of HGC were examined by in situ hybridization technique. Results Positive expression rates of gelatinase A in the normal gastric tissue, peritumor tissue and HGC were 8.3 %, 35.7% and 83.3% respectively ( P <0.01). The positive rates of gelatinase A in the group with serosal invasion and lymph node metastasis were 93.1% and 90.6%, much higher than those in the group with negative ones (42.9% and 25.0%) .By in situ hybridization, gelatinase A mRNA was showed to be expressed in the extracellular matrix of tumor tissues,which surrounded the invasive margin of cancer tissues. The positive cells at these sites were mainly tumor infiltrating macrophages. Conclusion There is good correlation between gelatinase A mRNA expression and the invasion, metastasis of HGC. So it can be used as a useful marker for invasion and metastasis of HGC.