利用组织芯片研究Rb基因在原发性肝细胞肝癌中的表达

Study on the Expression of Rb Gene in Primary Hepatocellular Carcinoma by Tissue Chip

目的 研究原发性肝细胞肝癌 (HCC)中Rb基因的表达。方法 利用组织芯片技术和免疫组织化学技术(SP法 )检测 40例HCC及其相应癌旁、远癌正常组织中Rb蛋白的表达情况。结果 40例原发性HCC患者标本中 ,肿瘤组织中Rb蛋白表达缺失者有 17例 ,癌旁组织中有 4例 ,在远癌正常组织有 1例 ,HCC肿瘤组织中Rb蛋白缺失率显著高于相应癌旁及远癌正常组织 (P<0 .0 5 ) ;Rb蛋白表达的缺失与肿瘤的大小、病理学分级无统计学相关性 (P>0 .0 5 )。结论 HCC的发生、发展与Rb蛋白的缺失可能有关。

Objective To determine whether Rb gene is involved in the genesis of primary hepatocellular carcinoma(HCC). Methods Forty paraffin specimens of primary HCCs with corresponding adjacent liver tissues and normal liver tissues were investigated for Rb protein expression by tissue chip and SP immunohistochemical technique. Results Loss of Rb protein expression occurred in 17 of 40 tumor samples, whereas in 4 of 40 adjacent liver tissue samples, only 1 of 40 normal liver tissue specimens showed negative Rb staining.Rb protein deletion in HCC was higher than that in adjacent tissues and normal liver tissues ( P <0.05).Rb protein deletion rate doesn't correlated remarkably with tumor size or phathology grade of HCC ( P >0.05). Conclusion Rb protein deletion may play an important role in the tumorigenesis of HCC.Tissue chip is an effective high throughput technique platform for the study of tumor molecular pathology.