家蝇幼虫血淋巴抗白念珠菌作用的初步研究

Preliminary studies of the antifungal activity of hemolymph of the housefly

目的 用微量液体培养生长抑制法检测家蝇幼虫血淋巴的抗白念珠菌活性。方法 用大肠杆菌接种家蝇 3期幼虫 ,收集接种后不同时间血淋巴 ,设立空白对照、药物对照和蛋白酶K对照组 ,检测血淋巴和血淋巴 80℃ 5min去粗蛋白后提取多肽对 3株白念珠菌的抗菌活性。结果 大肠杆菌诱导 2 4h后血淋巴出现抗白念珠菌活性 ,能被水解蛋白酶灭活 ,去粗蛋白后仍有活性 ,对氟康唑敏感株及耐药株白念珠菌均有抑制作用。

Aim To detect the antifungal activity of the induced hemolymph of housefly larva using liquid growth inhibition assays.Method Antifungal activity of the hemolymph induced with Escherichia coli was detected,compared with controls (negative,antifungal drug and the hemolymph digested by protease K.We also detected antifungal activity of the hemolymph after being heated by removing denatured protein at 80℃ 5min.Results The induced hemolymph had antifungal activity,which could be destroyed by protease K,and still could be detected after removing denatured protein from it,and could inhibite the growth of Candida albicans including both the sensitive strain and the resistent strain to fluconazole.Conclusion The induced hemolymph of housefly larva has antifungal activity.