不同超滤条件对四逆汤中乌头总碱、甘草酸及固含量的影响

Effect of ultrafiltration conditions on content of aconitines, glycyrrhizic acid and solid residue in Sini Decoction

目的 :探讨四逆水溶液 (A)、4 0 % (B)和 6 0 % (C)醇溶液分别经分子截留值为 10 0 0、30 0 0和 5 0 0 0的超滤膜超滤前后乌头总碱、甘草酸和固含量的变化。方法 :离子对萃取—分光光度法、以薄层—紫外分光光度法和重量法分别测定乌头总碱、甘草酸含量和干浸膏重量。结果 :乌头总碱和甘草酸的损失与超滤膜分子截留值和乙醇浓度成反比 ,甘草酸的损失均超过 10 % ,固含量的降低与甘草酸的损失成正比关系。结论 :四逆汤在水溶液和 4 0 %～ 6 0

Objective: To investigate the effect of ultrafiltration conditions on the content of aconitines, glycyrrhizic acid and solid residue in Sini Decoction water (A), 40% ethanol (B) and 60% ethanol (C) solution. Methods: Ion pair extraction combined with UV spectrophotometry, TLC combined with UV spectrophotometry and weighing method have been used respectively to detect the content of aconitines, glycyrrhizic acid and weight of dry extractum. Results: Results showed the losses of aconitines and glycyrrhizic acid were inversely proportional to the size of ultrafiltration membrane and the concentration of ethanol. The loss of glycyrrhizic acid was larger than 10%. The decrease of solid residue was proportional to the loss of glycyrrhizic acid. Conclusion: Ultrafiltration of Sini Decoction in water, 40%～60% ethanol solution cannot eliminate impurities.