放射复合伤口愈合中外周血淋巴细胞凋亡及其与愈合延迟的关系

APOPTOSIS OF PERIPHERAL LYMPHOCYTE IN RADIATION COMPOUND WOUND HEALING AND ITS RELATIONSHIP TO DELAYED-WOUND HEALING

为研究放射复合伤口愈合中外周血淋巴细胞凋亡规律并探讨其与愈合延迟的关系 ,将 12 0只大鼠随机分为单纯创伤组与创伤复合照射组 ,用原位末端标记 (TUNEL)方法检测细胞凋亡 ,碱磷酶免疫组织化学方法检测Bax、Bcl 2蛋白表达。结果发现 ,创伤复合照射组动物白细胞数出现下降 ,伤后 3天降至最低 ,伤后 5天仍显著低于单纯创伤组 ;伤后两组动物外周血淋巴细胞凋亡率均升高 ,但创伤照射组凋亡率始终高于单纯创伤组 ;伤后不同时间淋巴细胞Bax蛋白出现升高 ,创伤照射组明显高于单纯创伤组 ,而Bcl 2呈现相反的变化趋势。该结果提示 ,照射后外周血白细胞数的降低和淋巴细胞凋亡的增加是辐射延迟伤口愈合的重要原因 ;Bax和Bcl

To study the apoptotic regularity of peripheral lymphocytes in radiation compound wound healing and explore their relationship to radiation delayed wound healing, apoptosis and Bax and Bcl 2 proteins were estimated by in situ terminal labeling (TUNEL) and immunohistochemical method of alkaline phosphatase, respectively. White blood cells in radiation compound wound(RW) group decreased and reached the lowest 3 days after wound, it was still lower in RW group than in simple wound(SW) group 5 days after wound; apoptosis of peripheral lymphocytes of both groups increased, however, the lymphocyte apoptosis in RW group was higher than that in SW from beginning to end in the whole process of wound healing; the expression of Bax protein of lymphocyte increased at different times after wound, and the expression in RW group was obviously higher than that in SW group, while Bcl 2 showed an opposite tendency. It is suggested that reduction of white blood cells and increment of lymphocyte apoptosis are the major causes of radiation delayed wound healing; Bax and Bcl 2 proteins take part in the regulation of lymphocyte apoptosis in wound healing.