摘要
目的:研究幽门螺杆菌(Hp)感染的胃癌及癌前病变患者胃粘膜p53、bcl-2、c-myc基因的表达,以探讨Hp在胃癌发生、发展过程中的作用机制。方法:应用免疫组化方法测定60例胃癌组织、18例异型增生、29例肠上皮化生、35例萎缩性胃炎、29例浅表性胃炎组织中bcl-2、c-myc基因蛋白表达情况,PCR/SSCP测定p53基因第5~8外显子突变,Hp阳性由快速尿素酶法和HE染色确定。结果:胃癌组Hp阳性41例,阴性19例。在胃癌组织中p53、bcl-2、c-myc基因阳性表达分别为38例(63.3%)、38例(63.3%)、18例(30.0%)。p53、c-myc基因在胃癌中阳性表达率显著高于异型增生(P<0.05)。bcl-2在异型增生组织中的阳性表达率与胃癌、肠上皮化生相比无显著性差异(P>0.05)。p53、bcl-2、c-myc基因表达与胃癌分期、淋巴结转移均无显著性关系(P>0.05),但bcl-2与胃癌类型、分化程度显著相关(P<0.05)。每一种病变类型Hp阳性组p53、bcl-2、c-myc基因蛋白的阳性表达率均高于Hp阴性组,两者之间有显著性差异(P<0.05)。结论:Hp感染者具有更多肿瘤生物学行为,可以引起抑癌基因p53第5~8外显子突变,癌基因c-myc、凋亡调节基因bcl-2表达增加,Hp可能为促癌剂,在胃癌的发生发展中起一定作用。
Objective:To study the expression of tumor suppressive gene,regulatory gene of apoptosis and oncogene in gastric mucosa lesions of gastric cancer patients with Helicobacter pylori infection(Hp).Methods:Immunohistochemical staining was used to detect the expression of bcl-2,c-myc protein in60cases of gastric cancer,18dysplasia,29internal metaplasia,35at-rophic gastritis,29superficial gastritis.Exon5-8in the same tissues were detected by PCR,laser screen,PCR/SSCP.HP was assessed by using the rapid urease staining test combined with HE staining test.Results:There was no significant correlation for HP infection rate between gas-tric cancer and atrophic gastritis.The positive rate of p53,c-myc protein in gastric cancer was sig nificantly higher than that in dysplasia(P<0.05).The positive rate of bcl-2was not correlated between gastric cancer,intestinal metaplasia and dysplasia(P>0.05)but significantly higher than that in gastritis(P<0.05).Then expression rate of p53,bcl-2,c-myc in gastric can cer were38,(63.3%),38(63.3%),18(30.0%)respectively.No correlation between p53,bcl-2,cmyc and grade of pathologic stage,lymph node sta tus was ob served(P>0.05).There was sig nificant correla tion between bcl-2and type of gastric can cer,grade of differentiation(P<0.05).The expression rate of p53,bcl-2,c-myc in Hp positive group was higher than that in Hp neg ative group in same gas-tric disease.Conclusion:Hp might act as a tumor promoter in the gen esis of gastric cancer.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2002年第5期325-328,共4页
Chinese Journal of Clinical Oncology
基金
本文课题受吉林省卫生厅科研基金资助(编号:96072)