MMP-1、TIMP-1和U-PA在血管成形术后再狭窄中表达的实验研究

Experimental study for the expression of matrix metalloproteinase-1,tissue inhibitor of matrix metalloproteinase-1 and urokinase of plasminogen activator in restenosis after angioplasty

目的 本实验以兔髂动脉为模型 ,探讨基质金属蛋白酶 - 1 (MMP - 1 )、基质金属蛋白酶 - 1组织抑制剂 (TIMP- 1 )和尿激酶纤溶酶原激活剂 (U -PA)在血管成形术后再狭窄中的作用。方法 对 48只家兔髂动脉粥样硬化模型血管进行球囊扩张术 ,分别于术后不同时间行血管造影后处死动物 ,取扩张血管段用病理方法观察血管形态 ,用原位杂交法测定MMP- 1、TIMP - 1和U -PA的mRNA表达。结果 球囊扩张术后各时间点MMP - 1和TIMP - 1mRNA始终处于弱表达 ,没有明显升高 ;球囊扩张术后 1d ,U -PAmRNA在内膜和中膜表达增加 (++) ,4d时达高峰 (+++) ,7d后开始下降并保持弱表达(+)。结论 MMP - 1、TIMP - 1和U -PA在血管成形术后异常表达 ,为新生内膜形成的重要原因 ,可能在成形术后再狭窄中起重要作用。

Objective To investigate the function of MMP-1,TIMP-1and U-PA in restenosis after angioplasty. Methods The MMP-1, TIMP-1 and U-PA mRNA were detected by in situ hybridization in the rabbit iliac artery after balloon catheter injury at various time Results The expression of MMP-1,TIMP-1 mRNA keep low levels all the time after angioplasty and increased in atherosclerotic plaques,U-PA mRNA increased at one day,reached maximum on 4 days, decreased at 7 days and remained low levels Conclusion The abnormal expression of MMP-1,TIMP-1 and U-PA may play an important role in migration of vascular smooth muscle cells,formation of neointima and restinosis after angioplasty