阿拓莫兰对缺血再灌注损伤肝脏的保护作用

Protective effect of atomolan on liver during ischemia-reperfusion injury

目的 探讨阿拓莫兰对家兔肝缺血再灌注 (I/R)损伤肝脏的保护作用。方法 将 2 0只健康新西兰大耳白兔随机分为对照组、阿拓莫兰保护组。制备家兔肝I/R损伤模型 ,观察阿拓莫兰对谷丙转氨酶 (ALT)、谷草转氨酶 (AST)和肝组织黄嘌呤氧化酶 (XO)、超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH -PX)活性、丙二醛 (MDA)含量及肝细胞形态学变化的影响。结果 肝I/R损伤期间 ,ALT、AST、XO活性及MDA含量均明显升高 (P <0 0 5) ,SOD和GSH -PX活性显著下降 (P <0 0 5) ,肝细胞形态学发生异常变化。使用阿拓莫兰后 ,上述指标的异常变化显著减轻 ,其差异有显著意义 (P <0 0 5)。结论 阿拓莫兰通过清除氧自由基 (OFR) 。

Objective To explore the protective effect of atomolan in treating hepatic ischemia and reperfusion(I/R) injury.Methods Twenty rabbits were randomly divided into atomolan protection group and controlled group.Changes of several parameters,including alanine aminotransferase(ALT),aspartate aminotransferase(AST),xanthine oxidase(XO),malondiadehyde(MDA),glutathione peroxidase(GSH-PX) and superoxide dismutase(SOD) as well as liver morphological changes,and the effect of atomolan on them were observed during hepatic I/R injury.Results SOD and GSH-PX activity decreased significantly ( P <0 05),ALT, AST and XO activities as well as MDA concentration increased remarkably ( P <0 05),and abnormal morphological changes of hepatocytes were obvious during hepatic I/R injury;with treatment of atomolan,the abnormal changes of all above parameters were diminished remarkably ( P <0 05).Conclusion Atomolan possess preventive effects on hepatic I/R injury by eliminating oxygen free radicals(OFR).