长根菇与鸡從菌属间不对称融合后代特性研究

Study on the Characteristics of the Posterities Derived from Asymmetric Fusion Between Oudemansiella radicata and Termitomyces albuminosus

以长根菇和鸡菌为亲本进行属间不对称融合研究 ,从原生质体再生菌株中得到能够双核化的单核无锁状联合的菌株 2 0株。栽培结果显示 ,这类菌株不能形成子实体 ,且菌株间菌丝的生长差异显著。但在特定的培养条件下 ,单核菌株均可双核化形成双核菌株 ,双核菌株具有出菇能力 ,菌株间子实体颜色。

Through asymmetric fusion between O.radicata and T.albuminosus under high pH and high Ca 2+ conditions, 20 monokaryotic strains without clamp connection which could be dicaryotized were obtained from regenerative protoplast strains. The cultural test indicated that no fruitbody was developed from monokaryotic strains and the mycelium growth characteristics between monokaryotic strains were significantly different. Under given conditions, the monokaryotic strains were dicaryotized into dicaryotic strains which could form fruitbody. But the fruitbody colour, the stipe insertion mode and the mycelium growth rate of dicaryotized strains were significantly different from their parent strains and also significantly different between the dicaryotic strains tested.