红花的HPLC指纹图谱分析方法研究

Study on HPLC fingerprint analysis of Flos Carthami

目的 :用高效液相色谱法建立红花的指纹图谱分析方法。方法 :采用ODS色谱柱 ,流动相为甲醇 0 .4 %磷酸(50∶50 ) ,波长为 360nm。结果 :共有 9个色谱峰 (RSD≤ 3 % ,n =1 0 )。结论 :方法可靠、简便 。

Objective: To establish the method of fingerprint analysis on Flos Carthami by HPLC. Methods: The HPLC method was used, chromatography conditions were ODS column, CHOH 3 0.4%H 3PO 4 solution (50∶50) as mobile phase and 380nm at detective wavelength. Results: The result showed that 9 peaks (RSD≤3%, n=10 ) were common.Conclusion: This method is reliable, simple and provides a reference standard for the quality control of Flos Carthami.