类风湿性关节炎患者血清抗Ⅱ型胶原抗体的检测及意义

Detection and significance of serum antibodies against type Ⅱcollagen in patients with rheumatoid arthritis

目的 探讨类风湿性关节炎 (RA)患者血清抗Ⅱ型胶原 (CⅡ )抗体阳性的意义。方法 分别以人Ⅱ型胶原蛋白(HCⅡ )及牛Ⅱ型胶原蛋白CⅡ (BCⅡ )作为包被抗原进行间接ELISA法 ,检测 30例RA患者 ,对照组 (30例非RA的风湿病患者和 34例健康人 )血清抗CⅡ抗体。结果 以HCⅡ及BCⅡ作为包被抗原检测RA患者血清抗CⅡ抗体的阳性率分别约为 30 .0 %和 33.3% ;对照组血清中抗体阳性率均约为 1.6 % ;二者差异极其显著 (P <0 .0 1)。抗CⅡ抗体阳性的RA患者RF的阳性率高于抗CⅡ抗体阴性的RA患者。与HCⅡ和BCⅡ均发生阳性反应的血清 8例 ,均为早期RA患者。结论 抗CⅡ抗体可作为判断RA患者病情的辅助手段之一 。

Aim To explore the significance of serum antibodies against type Ⅱ collagen in the patients with rheumatoid arthritis(RA) . Methods Human type Ⅱ collagen ( HCⅡ) and bovine CⅡ(BCⅡ) were used as coating antigen respectively to detect the anti-CⅡ antibodies by indirect ELISA in 30 RA patients, 30 patients with other rheumatic diseases and 34 normal individuals. Results The positive rates of serum anti-CⅡ antibodies to HCⅡ or BCⅡ in the RA patients were 30.0% and 33.3% , respectively; whereas those in control groups were all 1.6%. The difference between RA patients and control group was very notable( P < 0.01) . In anti-CⅡ antibody positive RA-patients positive rate of RF was more than that in anti-CⅡ antibody-negative RA patients. The serums reacting with HCⅡ and BCⅡ were all from the early RA patients. Conclusion Detection of anti-CⅡ antibody may be used as a means for judging RA patients' conditions. The replacement of HCⅡ by BCⅡ can be used to study the immunological role of B cells in the pathogenesis of RA.