海藻硫酸多糖对巨噬细胞所致肿瘤细胞凋亡的调节作用

Regulation of SPS on apoptosis of tumor cells caused by macrophages

目的 研究海藻硫酸多糖 (sulfatedpolysaccharidesfromseaweed ,SPS)对巨噬细胞 (Mφ)所致HepG2细胞凋亡的调节作用。方法 用MTT比色法 ,检测Mφ产生的TNF、NO(尤其是NO)及SPS对HepG2细胞的细胞毒性作用。将Mφ与HepG2按一定比例共孵育后 ,检测培养上清中NO2 /NO3 及细胞中cGMP的含量 ;用透射电镜观察HepG2细胞凋亡的形态学改变。结果 SPS对HepG2细胞没有直接的细胞毒性 ,但可通过促进Mφ产生NO和TNF ,增强Mφ对HepG2细胞的细胞毒性 ,增加共孵育细胞体系中cGMP的含量 ,并可明显促进HepG2细胞的凋亡。 结论 SPS可间接增强Mφ对HepG2细胞的细胞毒性 ,并可明显促进HepG2细胞的凋亡。

Aim to study the regulation of sulfated polysaccharides from seaweed (SPS)on apoptosis of tumor cells caused by Mφs. Methods Cytotoxicities of NO and SPS to HepG2 cells were detected by MTT colorimetry. Contents of NO 2 /NO 3 and cGMP after cocultivation of Mφs with HepG2 cells in cultural supernatant were detected by Griess assay and radioimmunoassay, respectively. The phenotype of apoptotic HepG2 cells were observed under transmission electron microscope. Results SPS had no direct cytotoxicity to HepG2 cells, but it increased con tents of the cGMP and NO 2 /NO 3 and induced the release of TNF, and then procecded to enhance the cytotoxicity to HepG2 cells, resulting significant in cell apoptosis. Conclusion SPS can enhance NO and TNF production by Mφs, resulting in significant apoptosis of HepG2 cells.