螺旋藻多糖对小鼠移植性肿瘤化疗后造血恢复及相关细胞因子的影响(英文)

Effect of polysaccharide from spirulina platensis on hematopoietic recovery and related cytokines in mice with transplant tumor after treated by chemotherapy

目的 :研究螺旋藻多糖 (polysaccharidefromspirulinaplatensis,PSP)对小鼠移植性肿瘤化疗后造血恢复及相关细胞因子的影响。方法 :通过右前肢皮下接种肿瘤细胞形成小鼠移植性实体瘤 ,给予PSP连续灌胃 10d ,d 4给予环磷酰胺 (CTX)腹腔注射连续 3d。于d 11,分别观察了外周血细胞、骨髓有核细胞及CFU S计数 ,用紫外分光光度计检测骨髓DNA含量 ,用双抗体夹心ELISA法检测血清中IL 1、IL 3、GM CSF、TNF含量。结果 :CTX可造成明显骨髓抑制 ,而PSP则提升了外周血细胞、增加了骨髓中有核细胞计数和DNA含量以及促进了CFU S形成。此外 ,PSP还增加了血清中IL 1、IL 3、GM CSF、TNFα含量。结论 :PSP可能通过促进内源性细胞因子的分泌来实现其促进小鼠移植性肿瘤化疗损伤后的造血恢复。

AIM: To evaluate the effect of polysaccharide from spirulina platensis (PSP) on hematopoietic recovery and related cytokines in mice with transplant tumor after treated by chemotherapy. METHODS: The tumor cells were subcutaneously injected in the right forefoot to induce transplant solid tumor in mice. The mice were ig with PSP for 10 d and injected intraperitoneally with cyclophosphamide (CTX) for 3 d. On d 11, the count of peripheral blood cell, nucleated cell in bone marrow and CFU-S (Colony Forming Unit-Spleen) was observed; The content of DNA in bone marrow was inspected by UV-spectrophotometer; The cytokines in serums were inspected by double antibody sandwich ELISA. RESULTS: CTX could induce evident myelosuppression. But PSP could elevate the level of peripheral blood cell, increase nucleated cell and DNA in bone marrow, and promote CFU-S formation. In addition, PSP could also increase the content of IL-1, IL-3, GM-CSF, and TNF-α in serum. CONCLUSION: It is probably that PSP accelerates the hematopoietic recovery in mice with transplant tumor treated by chemotherapy by promoting endogenous cytokines secretion.