高效液相色谱法测定蒲杞宫泰颗粒中芍药苷的含量

Determinatin of Paeoniflorin in Puqi Gongtai Granules by HPLC

目的 :应用高效液相色谱法测定蒲杞宫泰颗粒中芍药苷的含量。方法 :采用Zorbax C1 8H3 7柱 (5 μm ,4.6mmid×2 5 0mm) ,乙腈 水 36 %醋酸 (75∶42 5∶0 4)为流动相 ,检测波长 2 30nm。结果 :线性范围为 0 0 2～ 0 1μg,r =0 9998,回收率为98 84% ,RSD =1 84%。结论 :该方法用于测定蒲杞宫泰颗粒中芍苷的含量简便、可靠、准确 ,可用于该制剂的质量控制。

Objective:To determine the contents of Paeoniflorin in Puqi Gongtai granules by high performance liquid chromatography.Methods:The method used Zorbax-C_ 18 column(5μm,4.6mmid×250mm),acetonitrile:water:36% acetate acid(75∶425∶0.4) as the mobile phase.The detection wavelength was set at 230nm.Results:The method of linear range was 0.02～0.1μg,r=0.9998.The average recovery of the method was 98.84%, RSD =1.84%.Conclusion:The method to determine the contents of Paeoniflorin in Puqi Gongtai granules is simple,reliable,accurate and can be applied to the quality control of the preparation.